ADAM9 Kit ELISA

N° du produit ABIN2859343

Kit ELISA Humain ADAM9, test Colorimetric pour la quantification de Humain ADAM9.

Aperçu rapide pour ADAM9 Kit ELISA (ABIN2859343)

Antigène: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Épitope: AA 29-697

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit ADAM9 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM9

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A29-D697

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A29-D697

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 disintegrin domain.

Tissue Specificity: Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. .

Plaque: Pre-coated

Protocole: human ADAM9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM9 has been precoated onto 96-well plates. Standards(NSO, A29-D697) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM9 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM9 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 7.54, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 363, Standard deviation: 20.69, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 594, Standard deviation: 36.23, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 176, Standard deviation: 11.44, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 380, Standard deviation: 28.12, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 658, Standard deviation: 51.98, CV(%): 7.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Autre désignation: ADAM9

Sujet:

Protein Function: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. .

Background: Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

Synonyms: Disintegrin and metalloproteinase domain-containing protein 9,ADAM 9,3.4.24.-,Cellular disintegrin-related protein,Meltrin-gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,ADAM9,KIAA0021, MCMP, MDC9, MLTNG,

Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 9

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

ID gène: 8754

UniProt: Q13443

Pathways: Cellular Response to Molecule of Bacterial Origin, SARS-CoV-2 Protein Interactome