BMP7 Kit ELISA

N° du produit ABIN3071450

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris BMP7.

Aperçu rapide pour BMP7 Kit ELISA (ABIN3071450)

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Épitope: AA 292-430

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Détail du produit BMP7 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-7

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: S292-H430

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Hamster

Sensibilité: <10pg/ml

Attributs du produit: Protein Name: Bone morphogenetic protein 7
Full Gene Name: bone morphogenetic protein 7

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-7 amount of sample captured in plate.

Procédure de l'essai:

20-25

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP7

Sujet:

Protein Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-i² superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Synonyms: Bone morphogenetic protein 7,BMP-7,Osteogenic protein 1,OP-1,Bmp7,Bmp-7, Op1,

Full Gene Name: Bone morphogenetic protein 7

Cellular Localisation: Secreted.

ID gène: 12162

UniProt: P23359

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance