Leptin Receptor Kit ELISA
Aperçu rapide pour Leptin Receptor Kit ELISA (ABIN3198464)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 62.5 pg/mL
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Fonction
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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Specificité
- Leptin R ELISA Kit, Human
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Ingrédients
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- Plate
- Standard
- Diluent
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Matériel non inclus
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- protect your eyes
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Stock
- 4 °C
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Stockage commentaire
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- Leptin Receptor (LEPR)
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Autre désignation
- Leptin R
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Sujet
- Leptin receptor (Leptin R) also known as OB-R or CD295 is a protein that in humans is encoded by the LEPR gene. It functions as a receptor for the fat cell-specific hormone leptin.
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ID gène
- 3953
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NCBI Accession
- NM_001003679
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UniProt
- P48357
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Pathways
- Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour
Antigène Voir toutes Leptin Receptor (LEPR) Kits ELISA
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