RAGE Kit ELISA
Aperçu rapide pour RAGE Kit ELISA (ABIN3198608)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 15.63 pg/mL
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Fonction
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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Specificité
- RAGE ELISA Kit, Human
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Ingrédients
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- Plate
- Standard
- Diluent
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Matériel non inclus
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- protect your eyes
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Stock
- 4 °C
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Stockage commentaire
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
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Autre désignation
- RAGE
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Sujet
- Receptor for advanced glycation end product (RAGE) is a 35 kDa transmembrane receptor of the immunoglobulin super family. Its name comes from its ability to bind advanced glycation endproducts (AGE). In view of its inflammatory function in innate immunity and its ability to detect a class of ligands through a common motif, RAGE is often referred to as a pattern recognition receptor.
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ID gène
- 177
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NCBI Accession
- NM_001136
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UniProt
- Q15109
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Pathways
- Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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