AXL Kit ELISA (AXL Receptor tyrosine Kinase)

Details for Product AXL ELISA Kit No. ABIN3199198, Fournisseur: Connectez-vous pour afficher
Antigène
  • JTK11
  • UFO
  • AI323647
  • Ark
  • Tyro7
  • Ufo
  • AXL receptor tyrosine kinase
  • Axl receptor tyrosine kinase
  • AXL
  • Axl
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
24
14
5
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
31.25-2000 pg/mL
Seuil minimal de détection
31.25 pg/mL
Application
ELISA
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Fonction This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse AXL.
Type d'échantillon Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité AXL, Mouse
Sensibilité 1.95 pg/mL
Ingrédients Plate, Standard, Diluent
Matériel non inclus Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
Plasmids, Primers & others Plasmids, Primers & others AXL products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation AXL (AXL ELISA Kit Extrait)
Sujet AXL receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, AXL and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. AXL functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival.
ID gène 26362
NCBI Accession NM_009465
UniProt Q00993
Pathways Signalisation RTK, Cellular Response to Molecule of Bacterial Origin
Volume d'échantillon 100 μL
Durée du test 3 - 4 h
Plaque Pre-coated
Restrictions For Research Use only
Buffer Buffer contains: 0.02 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Intended for research use only and are not for use in diagnostic or therapeutic procedures.
Treat all chemicals with caution because they can be potentially hazardous.
It is recommended that this product is handled only by persons who have been trained in laboratory techniques and in accordance with the principles of good laboratory practice. Wear personal protection equipment such as laboratory coat, safety glasses and gloves.
Avoid direct contact with skin or eyes. Wash immediately with water in the case of contact with skin or eyes. Avoid contact of skin or mucous membranes with kit reagents or specimens. See material safety data sheet(s) for specific advice.
Pure water or deionized water must be used for reagent preparation.
The Stop Solution provided with this kit is an acid solution. Wear personal protection equipment with caution.
Do not expose kit reagents to strong light during storage and incubation.
Rubber or disposable latex gloves should be worn while handling kit reagents or specimens.
Avoid contact of substrate solution with oxidizing agents and metal.
Avoid splashing or generation of aerosols.
Use disposable pipette tips and/or pipettes to avoid microbial or cross-contamination of reagents or specimens which may invalidate the test.
Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagent.
Exposure to acid inactivates the HRP and antibody conjugate.
Substrate solution must be warmed to room temperature prior to use.
Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour at 121.5 °C.
Liquid wastes not containing acid and neutralized waste may be mixed with sodium hypochlorite in volumes such that the final mixture contains 1.0 % sodium hypochlorite. Allow 30 minutes for effective decontamination. Liquid waste containing acid must be neutralized prior to the addition of sodium hypochlorite.
All reagents including microplate, samples, standards and working solution should be warmed to room temperature before use.
To obtain accurate results, using adhesive film to seal the plate during incubation is suggested.
It is recommended that all samples and standards be assayed in duplicate.
Avoid foaming when mixing or reconstituting solutions containing protein.
To avoid cross-contamination, use separate reservoirs for each reagent and change pipette tips between each standard, sample and reagent.
When using an automated plate washer, adding a 30 seconds soak period before washing step and/or rotating the plate between wash steps may improve assay precision.
When pipetting reagents, maintain a consistent order of addition from well-to-well.
Keep Substrate solution protected from direct strong light. Substrate Solution should turn to gradations of blue after a proper color development.
Read absorbance within 30 minutes after adding stop solution.
Take care not to scratch the inner surface of the microwells.
Stock 4 °C
Stockage commentaire Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
Images (Fournisseur)
ELISA image for AXL Receptor tyrosine Kinase (AXL) ELISA Kit (ABIN3199198) AXL Receptor tyrosine Kinase (AXL) ELISA Kit
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