ICAM1 Kit ELISA

N° du produit ABIN411281

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat ICAM1. Il y a 19 publications disponibles.

Aperçu rapide pour ICAM1 Kit ELISA (ABIN411281)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Épitope: AA 28-493

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit ICAM1 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat ICAM-1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q28-T493

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q28-T493

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.

Plaque: Pre-coated

Protocole: rat ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards(NSO, Q28-T493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ICAM-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat ICAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat ICAM-1 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 98.2, Standard deviation: 5.11, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 769, Standard deviation: 39.2, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1420, Standard deviation: 68.16, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 123, Standard deviation: 10.7, CV(%): 8.7
• Sample 2: n=24, Mean(pg/ml): 776, Standard deviation: 50.44, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 1435, Standard deviation: 91.84, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour ICAM1 Kit ELISA (ABIN411281)

Zhu, He, Liu, Zhang, Yang, Liu, Wang: "Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 243, Issue 1, pp. 87-95, (2018) (PubMed).

Gu, Ding, Tang, Lei, Jiang, Xu: "A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis." dans: Frontiers in pharmacology, Vol. 9, pp. 331, (2018) (PubMed).

Yang, Gao, Wu, Yu, Li, Meng, Li, Yan, Jin: "Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression." dans: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 1975-1982, (2017) (PubMed).

Chen, Chen, Li, Dong, Teng, Nong, Pan, Lv, Gao, Wu: "Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy." dans: Oncotarget, Vol. 8, Issue 67, pp. 111522-111534, (2017) (PubMed).

Wang, Liu, He, Lv, Du: "Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats." dans: BMC complementary and alternative medicine, Vol. 15, pp. 402, (2016) (PubMed).

Chen, Zhang, Ma, Huang, Shao, Wu, Wang: "Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." dans: PLoS ONE, Vol. 10, Issue 11, pp. e0143868, (2016) (PubMed).

Barros, Borges, Ferreira, Carmo, Rosado, Fouque, Mafra: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" dans: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).

Tian, Wang, Cao, Zhong, Tu, Xiao, He, Zhai: "Comparison of protective effects of safflor injection and extract of Ginkgo biloba on lung ischemia/reperfusion injury in rabbits." dans: Chinese journal of integrative medicine, Vol. 21, Issue 3, pp. 229-33, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." dans: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Jiang, Zhang, Liu, Lin, Sun, Shen, Yan, Xie: "EETs alleviate ox-LDL-induced inflammation by inhibiting LOX-1 receptor expression in rat pulmonary arterial endothelial cells." dans: European journal of pharmacology, Vol. 727, pp. 43-51, (2014) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." dans: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Jing, Wang, Zhang, Cao, Chang, Dong, Guo, Li: "Upregulation of ICAM-1 in diabetic rats after transient forebrain ischemia and reperfusion injury." dans: Journal of inflammation (London, England), Vol. 11, Issue 1, pp. 35, (2014) (PubMed).

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." dans: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Asgary, Kelishadi, Rafieian-Kopaei, Najafi, Najafi, Sahebkar: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." dans: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." dans: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Zhang, Yang, Zhang, Sun, Zou, Kong, Dong, Ye, Yu: "Peroxisome proliferator-activated receptor-gamma is expressed by rat peritoneal mesothelial cells: its potential role in peritoneal cavity local defense." dans: American journal of nephrology, Vol. 26, Issue 6, pp. 602-11, (2007) (PubMed).

Xiang, Qian, Zhou, Liu, Li: "Crocetin inhibits leukocyte adherence to vascular endothelial cells induced by AGEs." dans: Journal of ethnopharmacology, Vol. 107, Issue 1, pp. 25-31, (2006) (PubMed).

Yang, Rui, Lu, Li, Liu, Yan, Wang: "Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats." dans: Life sciences, Vol. 77, Issue 20, pp. 2529-39, (2005) (PubMed).

Devanand, Bowers, Hoffman, Sackeim: "Acute and subacute effects of ECT on plasma HVA, MHPG, and prolactin." dans: Biological psychiatry, Vol. 26, Issue 4, pp. 408-12, (1989) (PubMed).

Détail du antigène

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM1

Classe de substances: Viral Protein

Sujet:

Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .

Background: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.

Synonyms: Intercellular adhesion molecule 1,ICAM-1,Major group rhinovirus receptor,CD54,ICAM1,

Full Gene Name: Intercellular adhesion molecule 1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling