IGFBPI Kit ELISA

N° du produit ABIN411288

Le kit ELISA IGFBPI (ABIN411288) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain IGFBPI. Ce kit ELISA a été cité dans 2 publications.

Aperçu rapide pour IGFBPI Kit ELISA (ABIN411288)

Antigène: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Épitope: AA 26-259

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Détail du produit IGFBPI Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Human IGFBP-1 ELISA Kit to quantitate Human IGFBP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A26-N259

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.9%, 7.1%, 6.6%
Inter-Assay Precision (CV%): 5.4%, 8.8%, 8.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IGFBPI Kit ELISA (ABIN411288)

Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." dans: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." dans: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

Détail du antigène

Antigène: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Autre désignation: IGFBP1

Sujet:

Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

Gene Full Name: insulin like growth factor binding protein 1

ID gène: 3484

UniProt: P08833

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding