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IGFBPI Kit ELISA

IGFBPI Reactivité: Humain AA 26-259 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
N° du produit ABIN411288
  • Antigène Voir toutes IGFBPI Kits ELISA
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Épitope
    AA 26-259
    Reactivité
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: A26-N259
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <1pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: A26-N259
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Plaque
    Pre-coated
    Protocole
    human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO, A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 6.09, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 736, Standard deviation: 37.54, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 70.85, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 173, Standard deviation: 10.21, CV(%): 5.9
    • Sample 2: n=24, Mean(pg/ml): 667, Standard deviation: 42.02, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 1360, Standard deviation: 102, CV(%): 7.5
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." dans: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

    Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." dans: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

  • Antigène Voir toutes IGFBPI Kits ELISA
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Autre désignation
    IGFBP1 (IGFBPI Produits)
    Synonymes
    cb656 Kit ELISA, igfbp1 Kit ELISA, IGFBP-1 Kit ELISA, MGC68538 Kit ELISA, afbp Kit ELISA, ibp1 Kit ELISA, pp12 Kit ELISA, igf-bp25 Kit ELISA, higfbp-1 Kit ELISA, IGFBP1 Kit ELISA, LOC100223208 Kit ELISA, LOC100305121 Kit ELISA, AFBP Kit ELISA, IBP1 Kit ELISA, IGF-BP25 Kit ELISA, PP12 Kit ELISA, hIGFBP-1 Kit ELISA, IGFBA Kit ELISA, insulin like growth factor binding protein 1 Kit ELISA, insulin-like growth factor binding protein 1a Kit ELISA, insulin like growth factor binding protein 1 S homeolog Kit ELISA, insulin like growth factor binding protein 1 L homeolog Kit ELISA, insulin-like growth factor binding protein 1 Kit ELISA, IGFBP1 Kit ELISA, igfbp1a Kit ELISA, igfbp1.S Kit ELISA, igfbp1.L Kit ELISA, igfbp1 Kit ELISA, LOC100305121 Kit ELISA, Igfbp1 Kit ELISA
    Sujet

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. .

    Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

    Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Placental protein 12,PP12,IGFBP1,IBP1,

    Full Gene Name: Insulin-like growth factor-binding protein 1

    Cellular Localisation: Secreted.
    ID gène
    3484
    UniProt
    P08833
    Pathways
    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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