IL1A Kit ELISA

N° du produit ABIN411291

Détail du produit IL1A Kit ELISA

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Épitope: AA 113-271

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.7-300 pg/mL

Seuil minimal de détection: 4.7 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 alpha

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli,S113-A271

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <0.5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: S113-A271

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the IL-1 family.

Plaque: Pre-coated

Protocole: human IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1 alpha amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-1 alpha standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 54, Standard deviation: 1.84, CV(%): 3.4
• Sample 2: n=16, Mean(pg/ml): 147, Standard deviation: 7.497, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 221, Standard deviation: 9.28, CV(%): 4.2,
• Sample 1: n=24, Mean(pg/ml): 48, Standard deviation: 2.64, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 139, Standard deviation: 10.29, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 234, Standard deviation: 14.98, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL1A Kit ELISA (ABIN411291)

Sun, Shi, Zheng, Min, Yang, Li, Liao, Huang, Zhang, Xu, Zhu, Cui, Liu: "Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway." dans: Cell death & disease, Vol. 9, Issue 3, pp. 260, (2018) (PubMed).

Li, Fan, Ma, Cao, Gao, Liu, Li: "Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery." dans: Experimental and therapeutic medicine, Vol. 13, Issue 3, pp. 971-975, (2017) (PubMed).

Han, Wang, Zhang, Liu, Li, Wang, Song, Pang, Ouyang, Tang: "Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NF?B pathways." dans: Biology open, Vol. 4, Issue 5, pp. 661-5, (2015) (PubMed).

Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." dans: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

Wang, Ji, Li, Han, Guo, Song, Quan, Wang, Han, Pang, Ouyang, Tang: "Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NF?B pathways." dans: Scientific reports, Vol. 4, pp. 4905, (2014) (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." dans: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." dans: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).

Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." dans: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).

Détail du antigène

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL1A (IL1A Produits)

Synonymes: IL-1A Kit ELISA, IL1 Kit ELISA, IL1-ALPHA Kit ELISA, IL1F1 Kit ELISA, IL-6 Kit ELISA, Il-1a Kit ELISA, IL-1 alpha Kit ELISA, IL-1alpha Kit ELISA, L1A Kit ELISA, interleukin 1 alpha Kit ELISA, interleukin 6 Kit ELISA, IL1A Kit ELISA, IL6 Kit ELISA, Il1a Kit ELISA

Sujet:

Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18 kDa.

Synonyms: Interleukin-1 alpha,IL-1 alpha,Hematopoietin-1,IL1A,IL1F1,

Full Gene Name: Interleukin-1 alpha

Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

ID gène: 3552

UniProt: P01583

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints