CCL5 Kit ELISA

N° du produit ABIN411348

Le kit ELISA CCL5 (ABIN411348) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CCL5. Ce kit ELISA a été cité dans 4 publications.

Aperçu rapide pour CCL5 Kit ELISA (ABIN411348)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Épitope: AA 24-91

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Détail du produit CCL5 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Human Rantes ELISA Kit to quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: S24-S91

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 3 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.9%, 6.9%, 7%
Inter-Assay Precision (CV%): 7%, 7.5%, 7%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour CCL5 Kit ELISA (ABIN411348)

Zhong, Tong, Li, Yuan, Hu, Hu, Song: "Mesenchymal stem cells in inflammatory microenvironment potently promote metastatic growth of cholangiocarcinoma via activating Akt/NF-κB signaling by paracrine CCL5." dans: Oncotarget, Vol. 8, Issue 43, pp. 73693-73704, (2017) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." dans: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Zhao, Bu, Wang, Ma, Ma: "GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation." dans: PLoS ONE, Vol. 9, Issue 1, pp. e85058, (2014) (PubMed).

Li, Luo, Fan, Sun: "Dynamic changes in serum monocyte chemoattractant protein-1, and regulated upon activation, normal T cell expressed and secreted levels in patients with minor intracerebral hemorrhage." dans: Neurosciences (Riyadh, Saudi Arabia), Vol. 17, Issue 4, pp. 314-20, (2012) (PubMed).

Détail du antigène

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: CCL5

Sujet:

Background: Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Gene Full Name: C-C motif chemokine ligand 5

ID gène: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration