TGFB1 Kit ELISA

N° du produit ABIN411355

Détail du produit TGFB1 Kit ELISA

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Épitope: AA 279-390

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Mouse TGF beta 1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: A279-S390

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: A279-S390

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF beta1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TGF beta1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TGF beta 1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 114, Standard deviation: 5.47, CV(%): 4.8
• Sample 2: n=16, Mean(pg/ml): 240, Standard deviation: 12, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 483, Standard deviation: 17.9, CV(%): 3.7,
• Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 6.75, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 248, Standard deviation: 15.4, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 515, Standard deviation: 30.4, CV(%): 5.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TGFB1 Kit ELISA (ABIN411355)

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Li, Liu, Duan, Liu, Wang, Lian, Zhuang, Fan: "Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3593-3602, (2018) (PubMed).

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Wang, Liu, Wang, Yang, Li, Ding, Sun, Bai, Li: "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2305683, (2018) (PubMed).

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Détail du antigène

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: TGFB1 (TGFB1 Produits)

Synonymes: CED Kit ELISA, DPD1 Kit ELISA, LAP Kit ELISA, TGFB Kit ELISA, TGFbeta Kit ELISA, TGF-beta Kit ELISA, TGF-BETA-1 Kit ELISA, TGF-beta5 Kit ELISA, ced Kit ELISA, dpd1 Kit ELISA, lap Kit ELISA, tgf-beta Kit ELISA, tgfb Kit ELISA, tgfb5 Kit ELISA, tgfbeta Kit ELISA, TGF-beta1 Kit ELISA, TGFbeta1 Kit ELISA, Tgfb Kit ELISA, Tgfb-1 Kit ELISA, ai39657 Kit ELISA, tgfb1 Kit ELISA, wu:fb13a07 Kit ELISA, xx:ai39657 Kit ELISA, TGFB1 Kit ELISA, csd Kit ELISA, cdb1 Kit ELISA, cdg2 Kit ELISA, csd1 Kit ELISA, csd2 Kit ELISA, csd3 Kit ELISA, ebmd Kit ELISA, lcd1 Kit ELISA, bigh3 Kit ELISA, cdgg1 Kit ELISA, betaig-h3 Kit ELISA, TGFB4 Kit ELISA, transforming growth factor beta 1 Kit ELISA, transforming growth factor beta-1 Kit ELISA, transforming growth factor beta 1 L homeolog Kit ELISA, transforming growth factor, beta 1 Kit ELISA, transforming growth factor, beta 1a Kit ELISA, transforming growth factor beta induced L homeolog Kit ELISA, TGFB1 Kit ELISA, Tgfb1 Kit ELISA, tgfb1.L Kit ELISA, tgfb1a Kit ELISA, tgfbi.L Kit ELISA

Sujet:

Protein Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). .

Background: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Synonyms: Transforming growth factor beta-1,TGF-beta-1,Latency-associated peptide,LAP,Tgfb1,

Full Gene Name: Transforming growth factor beta-1

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 21803

UniProt: P04202

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints