FLT1 Kit ELISA

N° du produit ABIN411372

Le kit ELISA FLT1 (ABIN411372) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain FLT1. Ce kit ELISA a été cité dans 3 publications.

Aperçu rapide pour FLT1 Kit ELISA (ABIN411372)

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Épitope: AA 27-687

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA)

Détail du produit FLT1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human VEGFR1/FLT1 ELISA Kit to quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: S27-H687

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 30 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.1%, 7.8%, 4.6%
Inter-Assay Precision (CV%): 4.7%, 9.7%, 4.7%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour FLT1 Kit ELISA (ABIN411372)

Bozduman, Ersoy Evans, Karahan, Hayran, Akbiyik, Lay: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." dans: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).

Bakir, Bakir, Sari, Celik, Gozel, Engin: "Evaluation of the relationship between serum levels of VEGF and sVEGFR1 with mortality and prognosis in patients with Crimean-Congo hemorrhagic fever." dans: Journal of medical virology, Vol. 85, Issue 10, pp. 1794-801, (2013) (PubMed).

Liu, Li, Su, Huang, Luo: "Soluble Fms-like tyrosine kinase-1 expression inhibits the growth of multiple myeloma in nude mice." dans: Acta biochimica et biophysica Sinica, Vol. 39, Issue 7, pp. 499-506, (2007) (PubMed).

Détail du antigène

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Autre désignation: FLT1

Sujet:

Background: Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Gene Full Name: fms related receptor tyrosine kinase 1

ID gène: 2321

UniProt: P17948

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals