LTA Kit ELISA

N° du produit ABIN6720236

N° du produit (Fournisseur): koa0358

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain LTA.

Aperçu rapide pour LTA Kit ELISA (ABIN6720236)

Antigène: LTA (Lymphotoxin-alpha (LTA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Détail du produit LTA Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

N° du produit (Fournisseur): koa0358

Fournisseur: Rockland

Fonction: Human TNF beta Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 7.1%, 6.6%, 7.1%
Inter-Assay Precision (CV%): 7.3%, 7.4%, 8.3%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: TNF beta

Classe de substances: Chemical

Sujet:

Background: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Gene Full Name: lymphotoxin alpha

ID gène: 4049

NCBI Accession: NP_000586

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process