PAI1 Kit ELISA

N° du produit ABIN411404

Détail du produit PAI1 Kit ELISA

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Épitope: AA 22-402

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PAI-1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21
Immunogen sequence: S22-P402

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: S22-P402

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the serpin family.

Tissue Specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Plaque: Pre-coated

Protocole: human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PAI-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA, citrate ) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PAI-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1539, Standard deviation: 76.95, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 3736, Standard deviation: 235.4, CV(%): 6.3
• Sample 3: n=16, Mean(pg/ml): 6234, Standard deviation: 405.21, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 3914, Standard deviation: 293.55, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 7335, Standard deviation: 601.47, CV(%): 8.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour PAI1 Kit ELISA (ABIN411404)

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." dans: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Sun, Yang, Xi, Chen, Fu, Sun, Yin, Li, Liu, Qin, Liu, Hou: "Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling." dans: Cell death & disease, Vol. 9, Issue 2, pp. 122, (2018) (PubMed).

Sun, Song, Liu, Fang, Wang, Wang, Li, Tang, Wu, Qin, Wu, Gao, Chen, Hu: "Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study." dans: Journal of thrombosis and thrombolysis, Vol. 43, Issue 3, pp. 401-410, (2017) (PubMed).

Nargesi, Shalchi, Nargesi, Sadeghpour, Zarifkar, Mozaffari, Imani, Esteghamati, Nakhjavani: "The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria." dans: Cell stress & chaperones, Vol. 21, Issue 2, pp. 361-5, (2016) (PubMed).

Wu, Liu, Chen, Chen, Zhang: "Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia." dans: Medicine, Vol. 95, Issue 8, pp. e2779, (2016) (PubMed).

Chen, Lu, Yang, Lu, Chen, Zhang: "Suppression of plasminogen activator inhibitor-1 by inhaled nitric oxide attenuates the adverse effects of hyperoxia in a rat model of acute lung injury." dans: Thrombosis research, Vol. 136, Issue 1, pp. 131-8, (2015) (PubMed).

Détail du antigène

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Autre désignation: Plasminogen activator inhibitor-1 (PAI-1) (SERPINE1 Produits)

Synonymes: PAI Kit ELISA, PAI-1 Kit ELISA, PAI1 Kit ELISA, PLANH1 Kit ELISA, SERPINE1 Kit ELISA, si:ch211-138a11.1 Kit ELISA, Planh1 Kit ELISA, PAI1A Kit ELISA, Pai1 Kit ELISA, Pai1aa Kit ELISA, Planh Kit ELISA, RATPAI1A Kit ELISA, serpin family E member 1 Kit ELISA, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog Kit ELISA, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade E, member 1 Kit ELISA, SERPINE1 Kit ELISA, serpine1.L Kit ELISA, serpine1 Kit ELISA, Serpine1 Kit ELISA

Sujet:

Protein Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .

Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Synonyms: Plasminogen activator inhibitor 1,PAI,PAI-1,Endothelial plasminogen activator inhibitor,Serpin E1,SERPINE1,PAI1, PLANH1,

Full Gene Name: Plasminogen activator inhibitor 1

Cellular Localisation: Secreted.

ID gène: 5054

UniProt: P05121

Pathways: Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration