IL-18 Kit ELISA

N° du produit ABIN411405

Le kit ELISA IL-18 (ABIN411405) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain IL-18. Ce kit ELISA a été cité dans 8 publications.

Aperçu rapide pour IL-18 Kit ELISA (ABIN411405)

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Épitope: AA 33-197

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit IL-18 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Human IL-18 ELISA Kit to quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Y33-D197

Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.3%, 5.6%, 4.2%
Inter-Assay Precision (CV%): 7.4%, 6.4%, 5.8%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IL-18 Kit ELISA (ABIN411405)

Wang, Qin, Wang, Chen, Lang, Zheng, Gao, Chen, Zhong, Mu, Wu, Zhang, Zhao, Zhong: "Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection affects viral replication and host response." dans: Scientific reports, Vol. 8, Issue 1, pp. 2887, (2018) (PubMed).

Wan, Yuan, Liu, Xue: "miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells." dans: Experimental and therapeutic medicine, Vol. 15, Issue 3, pp. 2429-2435, (2018) (PubMed).

Zhao, Shen, Feng, He, Xu, Xie, Shi, Zhou, Pan, Wang, Guo, Qin: "Regulatory B cells induced by pancreatic cancer cell-derived interleukin-18 promote immune tolerance via the PD-1/PD-L1 pathway." dans: Oncotarget, Vol. 9, Issue 19, pp. 14803-14814, (2018) (PubMed).

Esfahani, Saidijam, Najafi, Goodarzi, Movahedian: "The effect of salusin-β on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)." dans: ARYA atherosclerosis, Vol. 14, Issue 1, pp. 1-10, (2018) (PubMed).

Trentini, Castellazzi, Cervellati, Manfrinato, Tamborino, Hanau, Volta, Baldi, Kostic, Drulovic, Granieri, Dallocchio, Bellini, Dujmovic, Fainardi: "Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients." dans: Disease markers, Vol. 2016, pp. 3672353, (2017) (PubMed).

Huang, Liu, Ma, Liao, Lu, Huang, Qin, Liu, Fang: "Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages." dans: Journal of medical virology, (2017) (PubMed).

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." dans: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

McCarthy: "Management. The name game." dans: Nursing times, Vol. 85, Issue 1, pp. 44-5, (1989) (PubMed).

Détail du antigène

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Autre désignation: IL18

Sujet:

Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Gene Full Name: interleukin 18

ID gène: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome