Angiotensin II Kit ELISA

N° du produit ABIN416068

Kit ELISA Angiotensin II Rat, Colorimetric test pour la quantification de Rat Angiotensin II et a été mentionné dans 30+ publications.

Aperçu rapide pour Angiotensin II Kit ELISA (ABIN416068)

Antigène: Angiotensin II (Ang II)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 24.69 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit Angiotensin II Kit ELISA

Seuil minimal de détection: 24.69 pg/mL

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Sensibilité: < 9.17 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour Angiotensin II Kit ELISA (ABIN416068)

Hu, Li, Wang, Hu, Zhang, Chen, Wu, Jia: "The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta remodeling." dans: Molecular medicine reports, Vol. 22, Issue 1, pp. 387-397, (2020) (PubMed).

Zhang, Shi, Li, Sun, Zhang, Yu, Wu: "BPA disrupts 17‑estradiol‑mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway." dans: Molecular medicine reports, Vol. 22, Issue 1, pp. 416-422, (2020) (PubMed).

Lin, Huang, Xing, Xu, Zhang, Chen, Ke, Song, Zeng: "Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats." dans: Biomedicine & pharmacotherapy, Vol. 129, pp. 110400, (2020) (PubMed).

Yan, Wu, Zhou, Chen, He, Zhang, Chen, Yao, Su: "Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats." dans: Scientific reports, Vol. 10, Issue 1, pp. 10829, (2020) (PubMed).

Luo, Yang, Wang, Xu, Yang: "(Pro)renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting the intrarenal renin-angiotensin system." dans: American journal of physiology. Renal physiology, Vol. 319, Issue 5, pp. F930-F940, (2020) (PubMed).

Zhang, Li, Men, Peng, Shao, Zhang: "Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats." dans: Toxicology mechanisms and methods, Vol. 29, Issue 4, pp. 263-275, (2019) (PubMed).

Yu, Wei, Weiss, Felder: "Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats." dans: Neuroscience, Vol. 381, pp. 46-58, (2019) (PubMed).

Liu, Song, Lu, Geng, Jiang, Wang, Zhang, Shan: "Effects of renal denervation on monocrotaline induced pulmonary remodeling." dans: Oncotarget, Vol. 8, Issue 29, pp. 46846-46855, (2018) (PubMed).

Liu, Zhang, Wei, Yuan, Xue, Hao, Xu, Wang, Chen: "ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in cardiomyocytes." dans: International journal of cardiology, Vol. 257, pp. 150-159, (2018) (PubMed).

Silva, Azevedo, Figueiredo, Moura Junior, Coelho Junior, Martinelli, Machado, Alzamora, Talvani: "Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection." dans: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 51, Issue 5, pp. e6690, (2018) (PubMed).

Liu, Liu, Xiao, Wang, Yao, Zeng, Yu, Guan, Wei, Peng, Zhu, Wang, Yang, Zhong, Chen: "Mesenchymal stem cell-derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system." dans: Journal of the American Society of Hypertension : JASH, Vol. 12, Issue 6, pp. 470-478, (2018) (PubMed).

Fang, Deng, Zhang, Lu, Su, Xu, Zhou, Wang, Ou, Wang, Yang: "Role of (pro)renin receptor in albumin overload-induced nephropathy in rats." dans: American journal of physiology. Renal physiology, Vol. 315, Issue 6, pp. F1759-F1768, (2018) (PubMed).

Wang, Qian, Zhao, Xing, Sun: "β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibition of renal fibroblast activation and fibrosis." dans: Journal of pharmacological sciences, Vol. 133, Issue 4, pp. 203-213, (2017) (PubMed).

Zhao, Fu, Yang, Dong, Yang, Sun, Hou: "Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro." dans: Life sciences, Vol. 145, pp. 213-23, (2016) (PubMed).

Motawi, Darwish, Hamed, El-Rigal, Naser: "A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats." dans: Molecular neurobiology, (2016) (PubMed).

Ahmad, Sattar, Rathore, Abdulla, Khan, Azam, Abdullah, Johns: "Up Regulation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol-Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats." dans: PLoS ONE, Vol. 11, Issue 3, pp. e0150137, (2016) (PubMed).

Zhang, Wang, Zhang, Zhu, Yu, Peng: "Pleiotropic effects of survivin in vascular endothelial cells." dans: Microvascular research, Vol. 108, pp. 10-6, (2016) (PubMed).

Giampá, Mônico-Neto, de Mello, Souza, Tufik, Lee, Koike, Dos Santos, Antonio, Serra, Tucci, Antunes: "Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training." dans: PLoS ONE, Vol. 11, Issue 11, pp. e0167029, (2016) (PubMed).

Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." dans: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, (2016) (PubMed).

Li, Zhang, Sun, Zhang, Han: "Angiotensin-(1-7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats." dans: Journal of the American Society of Hypertension : JASH, Vol. 9, Issue 11, pp. 865-77, (2015) (PubMed).

Détail du antigène

Antigène: Angiotensin II (Ang II)

Autre désignation: AngII

Sujet: Ang-II, Angiotensin-2

Pathways: Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity