S100B Kit ELISA

N° du produit ABIN416398

Kit ELISA S100B Rat, Colorimetric test pour la quantification de Rat S100B et a été mentionné dans 13+ publications.

Aperçu rapide pour S100B Kit ELISA (ABIN416398)

Antigène: S100B (S100 Calcium Binding Protein B (S100B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit S100B Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100B in Serum,Plasma,Tissue Homogenate,Biological Fluids

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein B (S100B).
No significant cross-reactivity or interference between S100 Calcium Binding Protein B (S100B) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between S100 Calcium Binding Protein B (S100B) and analogues was observed.

Sensibilité: < 12.7 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein B (S100B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein B (S100B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C/-20 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour S100B Kit ELISA (ABIN416398)

Weiss, Dhir, Collett, Reola, Kaplan, Minimo, Omert, Leung: "Effect of complement C1-esterase inhibitor on brain edema and inflammation after mild traumatic brain injury in an animal model." dans: Clinical and experimental emergency medicine, Vol. 7, Issue 2, pp. 87-94, (2020) (PubMed).

Ma, Zhao, Zhang, Chen: "Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway." dans: Brain research, Vol. 1687, pp. 129-136, (2019) (PubMed).

Ma, Wang, Fan, Zhang, Luo, Huang, Zhang, Li, Zhao, Li: "Protective Effects of Cornel Iridoid Glycoside in Rats After Traumatic Brain Injury." dans: Neurochemical research, Vol. 43, Issue 4, pp. 959-971, (2018) (PubMed).

Chen, Sun, Dong, Zhang, Zhou, Yu, Diao, Guo, Tian et al.: "Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory Response Through the Th17 Immune ..." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 46, Issue 3, pp. 1175-1188, (2018) (PubMed).

Sedaghat, Taab, Kiasalari, Afshin-Majd, Baluchnejadmojarad, Roghani: "Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms." dans: Biomedicine & pharmacotherapy, Vol. 87, pp. 200-208, (2017) (PubMed).

Chen, Wang, Diao, Dong, Sun, Liu, Wu: "Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β Signaling Pathway in Rats." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 43, Issue 4, pp. 1634-1647, (2017) (PubMed).

Tamaoki, Suzuki, Okada, Fukui, Isobe, Saito: "Development of an experimental rat model of hyperammonemic encephalopathy and evaluation of the effects of rifaximin." dans: European journal of pharmacology, Vol. 779, pp. 168-76, (2016) (PubMed).

Ekiz, Özdemir-Kumral, Erşahin, Tuğtepe, Öğünç, Akakın, Kıran, Özsavcı, Biber, Hakan, Yeğen, Şener, Toklu: "Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid." dans: Neurourology and urodynamics, Vol. 36, Issue 4, pp. 1061-1068, (2016) (PubMed).

Ren, Yu, Wu, Liao, Tang, Zhan, Ou: "The experiment research of neuroprotection of hypoperfusion postconditioning on cerebral ischemia." dans: The Journal of craniofacial surgery, Vol. 25, Issue 4, pp. e392-7, (2014) (PubMed).

Er?ahin, Özdemir, Özsavc?, Akak?n, Ye?en, Reiter, Sener: "Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder." dans: Journal of pineal research, Vol. 52, Issue 3, pp. 340-8, (2012) (PubMed).

Yang, Li, Ge, Mi, Wang, Sun: "Protective effects of naloxone in two-hit seizure model." dans: Epilepsia, Vol. 51, Issue 3, pp. 344-53, (2010) (PubMed).

Hariri, Moallem, Mahmoudi, Memar, Hosseinzadeh: "Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: protective effects of crocin and safranal." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 48, Issue 10, pp. 2803-8, (2010) (PubMed).

Er?ahin, Toklu, Erzik, Cetinel, Akakin, Velio?lu-O?ünç, Tetik, Ozdemir, Sener, Ye?en: "The anti-inflammatory and neuroprotective effects of ghrelin in subarachnoid hemorrhage-induced oxidative brain damage in rats." dans: Journal of neurotrauma, Vol. 27, Issue 6, pp. 1143-55, (2010) (PubMed).

Détail du antigène

Antigène: S100B (S100 Calcium Binding Protein B (S100B))

Autre désignation: S100B

Sujet: S100-B, NEF, S100Beta, S-100 protein subunit beta

UniProt: P04631

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins