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Albumin Kit ELISA

ALB Reactivité: Rat Colorimetric Competition ELISA Cell Culture Supernatant
N° du produit ABIN454012
  • Antigène Voir toutes Albumin (ALB) Kits ELISA
    Albumin (ALB)
    Reactivité
    • 10
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Seuil minimal de détection
    50 ng/mL
    Application
    ELISA
    Fonction
    The Alpha2-macroglobulin (rat)-ELISA is designed for the quantitative determination of Alpha2-macroglobulin (A2M) in rat body fluids and cell culture supernatants.
    Type d'échantillon
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    Attributs du produit
    The determination of A2M is carried out as competitive ELISA.
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  • Plaque
    Pre-coated
    Protocole
    A2M has been pre-coated onto a microplate. During incubation the binding of an anti-A2M(rat)-antibody to the wells is inhibited by A2M from samples/standards. The immobilized antibody is detected using a HRP-labelled secondary antibody. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the A2M concentration.
    Procédure de l'essai

    Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 60 min, room temperature with shaking
    Wash Incubation of detection antibody (HRP-labeled): 100 µL/well 60 min with shaking
    Wash Substrate incubation: 100 µL/well 20 min, room temperature
    Add stop solution: 100 µL/well Read at 450 nm

    Restrictions
    For Research Use only
  • Stock
    4 °C
  • Hartner, Menendez-Castro, Cordasic, Marek, Volkert, Klanke, Rascher, Hilgers: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" dans: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).

    Wenzel, Rajakumar, Haase, Geusens, Hubner, Schulz, Brewer, Roberts, Hubel, Herse, Hering, Qadri, Lindschau, Wallukat, Pijnenborg, Heidecke, Riemekasten, Luft, Muller, Lamarca, Dechend: "Angiotensin II Type 1 Receptor Antibodies and Increased Angiotensin II Sensitivity in Pregnant Rats." dans: Hypertension, Vol. 58, Issue 1, pp. 77-84, (2011) (PubMed).

    Hyvoenen, Saurus, Wasik, Heikkilae, Havana, Trokovic, Saleem, Holthoefer, Lehtonen: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." dans: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).

    Hartner, Cordasic, Menendez-Castro, Volkert, Yabu, Kupraszewicz-Hutzler, Rascher, Hilgers: "Lack of {alpha}8 integrin aggravates podocyte injury in experimental diabetic nephropathy." dans: American journal of physiology. Renal physiology, (2010) (PubMed).

    Mansour, Mostafa, Nagi, Khattab, Al-Shabanah: "Protective effect of aminoguanidine against nephrotoxicity induced by cisplatin in normal rats." dans: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Vol. 132, Issue 2, pp. 123-8, (2002) (PubMed).

    Huang, Richter, Breidenbach, Vallon: "Human C-peptide acutely lowers glomerular hyperfiltration and proteinuria in diabetic rats: a dose-response study." dans: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 365, Issue 1, pp. 67-73, (2002) (PubMed).

  • Antigène Voir toutes Albumin (ALB) Kits ELISA
    Albumin (ALB)
    Autre désignation
    Albumin (ALB Produits)
    Synonymes
    PRO0883 Kit ELISA, PRO0903 Kit ELISA, PRO1341 Kit ELISA, ALB Kit ELISA, CSA Kit ELISA, Alb-1 Kit ELISA, Alb1 Kit ELISA, Albza Kit ELISA, LOC100136344 Kit ELISA, alb-a Kit ELISA, alb-b Kit ELISA, albumin Kit ELISA, serum albumin 1 Kit ELISA, albumin S homeolog Kit ELISA, ALB Kit ELISA, Alb Kit ELISA, LOC100136344 Kit ELISA, alb.S Kit ELISA
    Sujet
    A2M is a main part of the globulin fraction of serum. The normal serum concentration is about 20-60 µg/ml.
    Pathways
    Lipid Metabolism
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