Albumin Kit ELISA

N° du produit ABIN454013

Détail du produit Albumin Kit ELISA

Antigène: Albumin (ALB)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Seuil minimal de détection: 0.1 µg/mL

Application: ELISA

Fonction: This ELISA allows quantification of albumin in mouse urine, so that an estimation of kidney function is possible.

Type d'échantillon: Urine

Analytical Method: Quantitative

Attributs du produit: Determination of albumin is carried out as direct competitive ELISA.

Information d'application

Plaque: Pre-coated

Protocole: Mouse albumin has been pre-coated onto a microtiter plate. During incubation binding of an enzyme-linked anti-albumin(mouse)-antibody is inhibited by albumin from samples/ standards. After washing away any unbound antibody a substrate solution is added. Developing colour correlated with the amount of antibody conjugate. Absorption at 450 nm is reverse proportional to albumin concentration.

Procédure de l'essai:

Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 90 min with shaking,120 min without shaking.
Wash Substrate incubation: 100 µL/well 10 min, room temperature.
Add stop solution 100 µL/well.
Read at 450 nm

Précision du teste: Intraassay precision (CV):(sample dilution 1:100, n=12) at 3.1 µg/ml: 14.3%at 30.4 µg/ml: 6.1% at 223 µg/ml: 5.8%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour Albumin Kit ELISA (ABIN454013)

Liebisch, Bondeva, Franke, Hause, Wolf: "Growth arrest specific 2-like protein 1 expression is upregulated in podocytes through advanced glycation end-products." dans: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2016) (PubMed).

Schordan, Grisk, Schordan, Miehe, Rumpel, Endlich, Giebel, Endlich: "OPN deficiency results in severe glomerulosclerosis in uninephrectomized mice." dans: American journal of physiology. Renal physiology, (2013) (PubMed).

Lee, Auger, Park, Kang, Oak, Kim, Schini-Kerth: "An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice." dans: PLoS ONE, Vol. 8, Issue 6, pp. e65227, (2013) (PubMed).

Hyvoenen, Saurus, Wasik, Heikkilae, Havana, Trokovic, Saleem, Holthoefer, Lehtonen: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." dans: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).

Heikkilae, Juhila, Lassila, Messing, Peraelae, Lehtonen, Lehtonen, Sjef Verbeek, Holthofer: "beta-Catenin mediates adriamycin-induced albuminuria and podocyte injury in adult mouse kidneys." dans: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2437-46, (2010) (PubMed).

Détail du antigène

Antigène: Albumin (ALB)

Autre désignation: Albumin (ALB Produits)

Synonymes: PRO0883 Kit ELISA, PRO0903 Kit ELISA, PRO1341 Kit ELISA, ALB Kit ELISA, CSA Kit ELISA, Alb-1 Kit ELISA, Alb1 Kit ELISA, Albza Kit ELISA, LOC100136344 Kit ELISA, alb-a Kit ELISA, alb-b Kit ELISA, albumin Kit ELISA, serum albumin 1 Kit ELISA, albumin S homeolog Kit ELISA, ALB Kit ELISA, Alb Kit ELISA, LOC100136344 Kit ELISA, alb.S Kit ELISA

Sujet: When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages. In examining nephrotoxic substances and therapeutic strategies regarding kidney damages, animal models are frequently used.

Pathways: Lipid Metabolism