Albumin Kit ELISA

N° du produit ABIN454014

Ce kit ELISA Boeuf (Vache) Albumin est un kit ELISA Colorimetric conçu pour quantifier Boeuf (Vache) Albumin.

Aperçu rapide pour Albumin Kit ELISA (ABIN454014)

Antigène: Albumin (ALB)

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Urine

Détail du produit Albumin Kit ELISA

Seuil minimal de détection: 50 ng/mL

Fonction: The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.

Analytical Method: Quantitative

Specificité: Albumin (human) <0.1%Albumin (mouse) <0.1%

Attributs du produit: The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.

Information d'application

Plaque: Pre-coated

Protocole: Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.

Procédure de l'essai:

Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
Wash Substrate incubation: 100 µL/well 20 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm

Précision du teste: Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Albumin (ALB)

Autre désignation: Albumin

Pathways: Lipid Metabolism