Albumin Kit ELISA
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- Antigène Voir toutes Albumin (ALB) Kits ELISA
- Albumin (ALB)
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Reactivité
- Boeuf (Vache)
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Seuil minimal de détection
- 50 ng/mL
- Application
- ELISA
- Fonction
- The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
- Type d'échantillon
- Urine
- Analytical Method
- Quantitative
- Specificité
- Albumin (human) <0.1%Albumin (mouse) <0.1%
- Attributs du produit
- The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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- Plaque
- Pre-coated
- Protocole
- Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
- Procédure de l'essai
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Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
Wash Substrate incubation: 100 µL/well 20 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm - Précision du teste
- Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène Voir toutes Albumin (ALB) Kits ELISA
- Albumin (ALB)
- Autre désignation
- Albumin (ALB Produits)
- Synonymes
- PRO0883 Kit ELISA, PRO0903 Kit ELISA, PRO1341 Kit ELISA, ALB Kit ELISA, CSA Kit ELISA, Alb-1 Kit ELISA, Alb1 Kit ELISA, Albza Kit ELISA, LOC100136344 Kit ELISA, alb-a Kit ELISA, alb-b Kit ELISA, albumin Kit ELISA, serum albumin 1 Kit ELISA, albumin S homeolog Kit ELISA, ALB Kit ELISA, Alb Kit ELISA, LOC100136344 Kit ELISA, alb.S Kit ELISA
- Pathways
- Lipid Metabolism
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