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Albumin Kit ELISA

ALB Reactivité: Boeuf (Vache) Colorimetric Competition ELISA Urine
N° du produit ABIN454014
  • Antigène Voir toutes Albumin (ALB) Kits ELISA
    Albumin (ALB)
    Reactivité
    • 10
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Boeuf (Vache)
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Seuil minimal de détection
    50 ng/mL
    Application
    ELISA
    Fonction
    The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
    Type d'échantillon
    Urine
    Analytical Method
    Quantitative
    Specificité
    Albumin (human) <0.1%Albumin (mouse) <0.1%
    Attributs du produit
    The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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  • Plaque
    Pre-coated
    Protocole
    Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
    Procédure de l'essai

    Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
    Wash Substrate incubation: 100 µL/well 20 min, room temperature.
    Add stop solution: 100 µL/well.
    Read at 450 nm

    Précision du teste
    Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
    Restrictions
    For Research Use only
  • Stock
    4 °C
  • Antigène Voir toutes Albumin (ALB) Kits ELISA
    Albumin (ALB)
    Autre désignation
    Albumin (ALB Produits)
    Synonymes
    PRO0883 Kit ELISA, PRO0903 Kit ELISA, PRO1341 Kit ELISA, ALB Kit ELISA, CSA Kit ELISA, Alb-1 Kit ELISA, Alb1 Kit ELISA, Albza Kit ELISA, LOC100136344 Kit ELISA, alb-a Kit ELISA, alb-b Kit ELISA, albumin Kit ELISA, serum albumin 1 Kit ELISA, albumin S homeolog Kit ELISA, ALB Kit ELISA, Alb Kit ELISA, LOC100136344 Kit ELISA, alb.S Kit ELISA
    Pathways
    Lipid Metabolism
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