SFRP2 Kit ELISA

N° du produit ABIN455159

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris SFRP2.

Aperçu rapide pour SFRP2 Kit ELISA (ABIN455159)

Antigène: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit SFRP2 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse Free-T3 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse Free-T3.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 0.078 pmol/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Secreted frizzled-related protein 2,sFRP-2,Protein SDF5,Secreted apoptosis-related protein 1,SARP-1,Sfrp2,Sarp1,Sdf5

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Free-T3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Free-T3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Free-T3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Autre désignation: Sfrp2

Pathways: Signalisation WNT, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation