IL-4 Kit ELISA

N° du produit ABIN455592

Le kit ELISA IL-4 (ABIN455592) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris IL-4.

Aperçu rapide pour IL-4 Kit ELISA (ABIN455592)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit IL-4 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse IL-4 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse IL-4.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,B-cell stimulatory factor 1,BSF-1,IGG1 induction factor,Lymphocyte stimulatory factor 1,Il4,Il-4

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Il4

Sujet: Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes,mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro. The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15. A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the recently cloned IL-13 R, has also been proposed. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13.

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation