IL-5 Kit ELISA

N° du produit ABIN455597

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris IL-5.

Aperçu rapide pour IL-5 Kit ELISA (ABIN455597)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit IL-5 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse IL-5 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse IL-5.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Interleukin-5,IL-5,B-cell growth factor II,BCGF-II,Cytotoxic T-lymphocyte inducer,Eosinophil differentiation factor,T-cell replacing factor,TRF,Il5,Il-5

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a 2 wavelength of 450 nm 2 nm. The concentration of IL-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: Il5

Sujet: IL-5 is a 135 amino acid (aa) polypeptide with a predicted mass of 12.5 kDa. It is secreted by a restricted number of mesenchymal cell types. In its native state, mature IL-5 is synthesized as a 115 aa, highly glycosylated 22 kDa monomer that forms a 40-50 kDa disulfide-linked homodimer. Although the content of carbohydrate is high, carbohydrate is not needed for bioactivity. Monomeric IL-5 has no activity and requires a homodimer for function, which is in contrast to the receptor-related IL-3 and GM-CSF cytokines that exist only as monomers. Just as one IL-3 and GM-CSF monomer binds to one receptor, one IL-5 homodimer is able to engage only one IL-5 receptor. It has been suggested that IL-5 (as a dimmer) undergoes a general conformational change after binding to one receptor molecule and this change precludes binding to a second receptor. Mouse and mouse mature IL-5 are 71% identical at the aa level. IL-5 appears to perform a number of functions on eosinophils including growth and differentiation, the down modulation of Mac-1, the upregulation of receptors for IgA and IgG, the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release. IL-5 may act in an adjunct fashion plays, however, the exact role is unclear. Finally, there is a great deal of interest in the interaction between IL-5 and the CC chemokine eotaxin. Studies suggest that inflammatory-induced and locally produced IL-5 and eotaxin may act on the bone marrow in a cooperative manner.

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour