LIF Kit ELISA

N° du produit ABIN455685

Détail du produit LIF Kit ELISA

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: This immunoassay kit allows for the specific measurement of human LIF concentrations in cell culture supernates, serum, and plasma.

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human LIF.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Attributs du produit: Homo sapiens,Human,Leukemia inhibitory factor,LIF,Differentiation-stimulating factor,D factor,Melanoma-derived LPL inhibitor,MLPLI,LIF,HILDA

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for LIF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF (LIF Produits)

Synonymes: CDF Kit ELISA, DIA Kit ELISA, HILDA Kit ELISA, MLPLI Kit ELISA, LIF, interleukin 6 family cytokine Kit ELISA, leukemia inhibitory factor Kit ELISA, LIF Kit ELISA, Lif Kit ELISA

Sujet: Leukemia Inhibitory Factor (or LIF) is a variably glycosylated, 38 - 67 kDa polypeptide originally identified as a proliferation inhibitor and differentiation inducer of the mouse M1 myeloid leukemia cell line. The mature LIF molecule measures 180 amino acid (aa) residues in length, with multiple potential N-linked and O-linked glycosylation sites plus six conserved cysteines that form three intramolecular disulfide bridges. Mature mouse LIF is 78% identical to human LIF at the aa sequence level. Although such homology might suggest a high conservation of LIF biology, notable differences exist between the reported forms of mouse and human LIF proteins and their receptors. For example, alternative splice events are known to occur in mice, but not humans, creating two isoforms of secreted LIF. Also, three isoforms of the LIF receptor a-chain, two soluble forms and one transmembrane form, have been reported in mice, but not in humans. Based on its helical structure, LIF is considered to be a member of the Interleukin-6 family of cytokines. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts , keratinocytes, regenerating skeletal muscle, mast cells , and fibroblasts.

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding