Prolactin Kit ELISA

N° du produit ABIN456250

Kit ELISA Prolactin Souris, Colorimetric test pour la quantification de Souris Prolactin.

Aperçu rapide pour Prolactin Kit ELISA (ABIN456250)

Antigène: Prolactin (PRL)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Prolactin Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse prolactin, PRL concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse prolactin.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Prolactin,PRL,Prl

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to prolactin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for prolactin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain prolactin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: Prolactin (PRL)

Autre désignation: Prl

Sujet: Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of prolactin is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition factors.

Pathways: Signalistation JAK/STAT, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus