F2RL1 Kit ELISA
Aperçu rapide pour F2RL1 Kit ELISA (ABIN456689)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.156 ng/mL
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Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human TPS, concentrations in serum, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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Specificité
- This assay recognizes recombinant and natural human TPS.
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Réactivité croisée (Details)
- No significant cross-reactivity or interference was observed.
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Sensibilité
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< 0.036 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
Attributs du produit
- Homo sapiens,Human,Proteinase-activated receptor 2,PAR-2,Coagulation factor II receptor-like 1,G-protein coupled receptor 11,Thrombin receptor-like 1,F2RL1,GPR11,PAR2
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Protocole
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TPS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
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Autre désignation
- F2RL1
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
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