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IGFBP2 Kit ELISA
IGFBP2
Reactivité: Chien
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4881520
Détail du produit IGFBP2 Kit ELISA
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Antigène
Voir toutes IGFBP2 Kits ELISA
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Reactivité
Toutes les réactivités sur IGFBP2 Kits ELISA
Chien
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Canine (dog) Insulin-like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Canine Insulin-like Growth Factor Binding Protein 2 (IGFBP2)
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IGFBP2 Kits ELISA
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Autre désignation
IGFBP-2 (IGFBP2 Produits )
Synonymes
IBP2 Kit ELISA, IGF-BP53 Kit ELISA, AI255832 Kit ELISA, IBP-2 Kit ELISA, Igfbp-2 Kit ELISA, mIGFBP-2 Kit ELISA, IGFBP-2 Kit ELISA, ILGFBPA Kit ELISA, pIGFBP-2 Kit ELISA, igfbp2 Kit ELISA, MGC65741 Kit ELISA, MGC76823 Kit ELISA, MGC174445 Kit ELISA, IGFBP2 Kit ELISA, ibp2 Kit ELISA, igf-bp53 Kit ELISA, igfbp2a Kit ELISA, si:ch211-2k18.3 Kit ELISA, insulin like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2a Kit ELISA, insulin-like growth factor binding protein 2b Kit ELISA, IGFBP2 Kit ELISA, Igfbp2 Kit ELISA, igfbp2a Kit ELISA, igfbp2 Kit ELISA, igfbp2b Kit ELISA
Pathways
Myometrial Relaxation and Contraction , Growth Factor Binding , Activated T Cell Proliferation
Vus récemment
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