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ANGPTL3 Kit ELISA

ANGPTL3 Reactivité: Humain Colorimetric Sandwich ELISA 8.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4881825
  • Antigène Voir toutes ANGPTL3 Kits ELISA
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Reactivité
    • 5
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    • 2
    • 2
    • 1
    • 1
    • 1
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    8.2-2000 pg/mL
    Seuil minimal de détection
    8.2 pg/mL
    Application
    ELISA
    Fonction
    Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples200 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Bassyouni, Sharaf, Wali, Mansour: "Clinical significance of Angiopoietin-1 in Behcet's disease patients with vascular involvement." dans: Heart and vessels, Vol. 31, Issue 6, pp. 918-24, (2017) (PubMed).

  • Antigène Voir toutes ANGPTL3 Kits ELISA
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Autre désignation
    ANGPTL3 (ANGPTL3 Produits)
    Synonymes
    fb60e11 Kit ELISA, fb66h02 Kit ELISA, wu:fb60e11 Kit ELISA, wu:fb66h02 Kit ELISA, zgc:111943 Kit ELISA, ANGPTL3 Kit ELISA, LOC100230785 Kit ELISA, ANG-5 Kit ELISA, ANGPT5 Kit ELISA, ANL3 Kit ELISA, FHBL2 Kit ELISA, hypl Kit ELISA, angiopoietin-like 3 Kit ELISA, angiopoietin like 3 Kit ELISA, angptl3 Kit ELISA, ANGPTL3 Kit ELISA, Angptl3 Kit ELISA
    ID gène
    27329
    UniProt
    Q9Y5C1
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