BIRC2 Kit ELISA

N° du produit ABIN4882354

Détail du produit BIRC2 Kit ELISA

Antigène: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human cIAP-1 (BIRC2/hIAP-2) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Autre désignation: cIAP-1 (BIRC2 Produits)

Synonymes: API1 Kit ELISA, HIAP2 Kit ELISA, Hiap-2 Kit ELISA, MIHB Kit ELISA, RNF48 Kit ELISA, c-IAP1 Kit ELISA, cIAP1 Kit ELISA, Api2 Kit ELISA, rIAP1 Kit ELISA, IAP1 Kit ELISA, ITA Kit ELISA, AW146227 Kit ELISA, Api1 Kit ELISA, Birc3 Kit ELISA, HIAP1 Kit ELISA, IAP2 Kit ELISA, MIAP1 Kit ELISA, MIAP2 Kit ELISA, MIHC Kit ELISA, cIAP2 Kit ELISA, mcIAP1 Kit ELISA, ciap-1 Kit ELISA, birc3 Kit ELISA, api1 Kit ELISA, birc2 Kit ELISA, c-iap1 Kit ELISA, ciap1 Kit ELISA, hiap-2 Kit ELISA, hiap2 Kit ELISA, mihb Kit ELISA, rnf48 Kit ELISA, baculoviral IAP repeat containing 2 Kit ELISA, baculoviral IAP repeat-containing 2 Kit ELISA, baculoviral IAP repeat containing 2 L homeolog Kit ELISA, BIRC2 Kit ELISA, Birc2 Kit ELISA, birc2 Kit ELISA, birc2.L Kit ELISA

ID gène: 329

UniProt: Q13490

Pathways: Apoptose, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades