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DFFA Kit ELISA

DFFA Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882605
  • Antigène Voir toutes DFFA Kits ELISA
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human DFF45/ICAD ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human S100 A11.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes DFFA Kits ELISA
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Autre désignation
    DFF45 (DFFA Produits)
    Synonymes
    DFF-45 Kit ELISA, DFF1 Kit ELISA, ICAD Kit ELISA, A330085O09Rik Kit ELISA, DFF35 Kit ELISA, Dff45 Kit ELISA, ICAD-L Kit ELISA, ICAD-S Kit ELISA, DFFA Kit ELISA, zgc:92229 Kit ELISA, DNA fragmentation factor subunit alpha Kit ELISA, DNA fragmentation factor, alpha subunit Kit ELISA, DNA fragmentation factor, 45kDa, alpha polypeptide Kit ELISA, DNA fragmentation factor, alpha polypeptide Kit ELISA, DFFA Kit ELISA, Dffa Kit ELISA, dffa Kit ELISA
    ID gène
    1676
    UniProt
    O00273
    Pathways
    Apoptose, Caspase Cascade in Apoptosis
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