HNF4A Kit ELISA

N° du produit ABIN4883203

Détail du produit HNF4A Kit ELISA

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human HNF-4 alpha/NR2A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Autre désignation: HNF-4 alpha (HNF4A Produits)

Synonymes: HNF4A Kit ELISA, HNF4alpha Kit ELISA, CG9310 Kit ELISA, DmHNF4 Kit ELISA, Dmel\\CG9310 Kit ELISA, HNF-4 Kit ELISA, HNF-4(D) Kit ELISA, HNF4 Kit ELISA, Hnf-4 Kit ELISA, Hnf-4h Kit ELISA, NR2A4 Kit ELISA, dHNF-4 Kit ELISA, dHNF4 Kit ELISA, Hnf4 Kit ELISA, Hnf4alpha Kit ELISA, MODY1 Kit ELISA, Nr2a1 Kit ELISA, Tcf14 Kit ELISA, HNF4a7 Kit ELISA, HNF4a8 Kit ELISA, HNF4a9 Kit ELISA, MODY Kit ELISA, NR2A1 Kit ELISA, NR2A21 Kit ELISA, TCF Kit ELISA, TCF14 Kit ELISA, HNF-4alpha Kit ELISA, fb58h09 Kit ELISA, id:ibd1279 Kit ELISA, wu:fb58h09 Kit ELISA, hnf4 Kit ELISA, nr2a1 Kit ELISA, hepatocyte nuclear factor 4 alpha Kit ELISA, Hepatocyte nuclear factor 4 Kit ELISA, hepatic nuclear factor 4, alpha Kit ELISA, hepatocyte nuclear factor 4, alpha Kit ELISA, hepatocyte nuclear factor 4 alpha S homeolog Kit ELISA, HNF4A Kit ELISA, hnf4a Kit ELISA, Hnf4 Kit ELISA, Hnf4a Kit ELISA, hnf4a.S Kit ELISA

ID gène: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process