LIFR Kit ELISA

N° du produit ABIN4883608

Détail du produit LIFR Kit ELISA

Antigène: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human LIF R alpha ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes human LIF R alpha. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Autre désignation: LIF R alpha (LIFR Produits)

Synonymes: A230075M04Rik Kit ELISA, AW061234 Kit ELISA, LIF Kit ELISA, CD118 Kit ELISA, LIF-R Kit ELISA, SJS2 Kit ELISA, STWS Kit ELISA, SWS Kit ELISA, leukemia inhibitory factor receptor Kit ELISA, LIF receptor alpha Kit ELISA, leukemia inhibitory factor receptor alpha Kit ELISA, Lifr Kit ELISA, LOC397451 Kit ELISA, LIFR Kit ELISA

ID gène: 3977

UniProt: P42702

Pathways: Signalistation JAK/STAT, Growth Factor Binding