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Neuropilin 1 Kit ELISA

NRP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883993
  • Antigène Voir toutes Neuropilin 1 (NRP1) Kits ELISA
    Neuropilin 1 (NRP1)
    Reactivité
    • 6
    • 4
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Neuropilin-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Neuropilin 1 (NRP1) Kits ELISA
    Neuropilin 1 (NRP1)
    Autre désignation
    Neuropilin-1 (NRP1 Produits)
    Synonymes
    NRP1 Kit ELISA, cd304 Kit ELISA, neuropilin Kit ELISA, np-1 Kit ELISA, npn-1 Kit ELISA, nrp Kit ELISA, nrp-1 Kit ELISA, vegf165r Kit ELISA, BDCA4 Kit ELISA, CD304 Kit ELISA, NP1 Kit ELISA, NRP Kit ELISA, VEGF165R Kit ELISA, C530029I03 Kit ELISA, NP-1 Kit ELISA, NPN-1 Kit ELISA, Npn1 Kit ELISA, Nrp Kit ELISA, neuropilin 1 Kit ELISA, neuropilin 1 L homeolog Kit ELISA, NRP1 Kit ELISA, nrp1 Kit ELISA, Nrp1 Kit ELISA, nrp1.L Kit ELISA
    ID gène
    8829
    UniProt
    O14786
    Pathways
    Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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