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PLA2G4A Kit ELISA
PLA2G4A
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884173
Détail du produit PLA2G4A Kit ELISA
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Antigène
Voir toutes PLA2G4A Kits ELISA
PLA2G4A
(Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
Reactivité
Toutes les réactivités sur PLA2G4A Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human PLA2G4A ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes PLA2G4A Kits ELISA
PLA2G4A
(Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
Autre désignation
PLA2G4A (PLA2G4A Produits )
Synonymes
PLA2G4 Kit ELISA, cPLA2-alpha Kit ELISA, Pla2c Kit ELISA, Pla2g4 Kit ELISA, cPLA2 Kit ELISA, pla2g4 Kit ELISA, CPLA2 Kit ELISA, PLA2G4A Kit ELISA, PLA2 Kit ELISA, PLA2G1B Kit ELISA, cPLA2alpha Kit ELISA, pla2g4a Kit ELISA, si:dkey-97o5.1 Kit ELISA, phospholipase A2 group IVA Kit ELISA, phospholipase A2 group IVA L homeolog Kit ELISA, phospholipase A2, group IVA (cytosolic, calcium-dependent) Kit ELISA, phospholipase A2, group IVAb (cytosolic, calcium-dependent) Kit ELISA, PLA2G4A Kit ELISA, Pla2g4a Kit ELISA, pla2g4a.L Kit ELISA, pla2g4ab Kit ELISA
ID gène
5321
UniProt
P47712
Pathways
Inositol Metabolic Process , G-protein mediated Events , VEGF Signaling
Vus récemment
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