TXN Kit ELISA

N° du produit ABIN4884884

Détail du produit TXN Kit ELISA

Antigène: TXN (Thioredoxin (TXN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Thioredoxin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Thioredoxin-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TXN (Thioredoxin (TXN))

Autre désignation: Thioredoxin-1 (TXN Produits)

Synonymes: TRDX Kit ELISA, TRX Kit ELISA, TRX1 Kit ELISA, ADF Kit ELISA, AW550880 Kit ELISA, Trx1 Kit ELISA, Txn Kit ELISA, trx Kit ELISA, TXN Kit ELISA, SCM1.18 Kit ELISA, trxA3 Kit ELISA, thioredoxin Kit ELISA, TR-1 Kit ELISA, Trx-1 Kit ELISA, thioredoxin Kit ELISA, thioredoxin 1 Kit ELISA, thioredoxin L homeolog Kit ELISA, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Kit ELISA, thioredoxin pseudogene Kit ELISA, cytosolic thioredoxin Trx1 Kit ELISA, TXN Kit ELISA, Txn1 Kit ELISA, txn.L Kit ELISA, txn Kit ELISA, trx Kit ELISA, SCO0885 Kit ELISA, PHG25ORF099c Kit ELISA, LOC100349046 Kit ELISA, Txn Kit ELISA, trx1 Kit ELISA

ID gène: 7295

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis