CD59 Kit ELISA

N° du produit ABIN4885162

Détail du produit CD59 Kit ELISA

Antigène: CD59

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human CD59 SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CD59

Autre désignation: CD59 (CD59 Produits)

Synonymes: 16.3A5 Kit ELISA, 1F5 Kit ELISA, EJ16 Kit ELISA, EJ30 Kit ELISA, EL32 Kit ELISA, G344 Kit ELISA, HRF-20 Kit ELISA, HRF20 Kit ELISA, MAC-IP Kit ELISA, MACIF Kit ELISA, MEM43 Kit ELISA, MIC11 Kit ELISA, MIN1 Kit ELISA, MIN2 Kit ELISA, MIN3 Kit ELISA, MIRL Kit ELISA, MSK21 Kit ELISA, p18-20 Kit ELISA, Cd59a Kit ELISA, Cd59b Kit ELISA, MACIP Kit ELISA, CD59 molecule (CD59 blood group) Kit ELISA, CD59 molecule Kit ELISA, CD59 Kit ELISA, Cd59 Kit ELISA

ID gène: 966

UniProt: P13987

Pathways: Système du Complément