IGF1R Kit ELISA

N° du produit ABIN4885550

Détail du produit IGF1R Kit ELISA

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Mouse IGF-1R ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Mouse IGF-1R .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: IGF-1 R (IGF1R Produits)

Synonymes: CMT2N Kit ELISA, CD221 Kit ELISA, IGFIR Kit ELISA, IGFR Kit ELISA, JTK13 Kit ELISA, IGFIRC Kit ELISA, IGFR1 Kit ELISA, IGF-1R Kit ELISA, IGF1R Kit ELISA, DKFZp469I0618 Kit ELISA, igf-1r Kit ELISA, xIGF-1R Kit ELISA, xIGFR Kit ELISA, xigf1r Kit ELISA, A330103N21Rik Kit ELISA, D930020L01 Kit ELISA, hyft Kit ELISA, igf-ir Kit ELISA, IGF-IR Kit ELISA, IGF-IRb Kit ELISA, wu:fb03h06 Kit ELISA, IGF-IRa Kit ELISA, sb:eu1090 Kit ELISA, wu:fi20b10 Kit ELISA, alanyl-tRNA synthetase Kit ELISA, insulin like growth factor 1 receptor Kit ELISA, insulin-like growth factor 1 receptor Kit ELISA, insulin-like growth factor 1 Kit ELISA, insulin-like growth factor I receptor Kit ELISA, insulin like growth factor 1 receptor S homeolog Kit ELISA, insulin-like growth factor 1b receptor Kit ELISA, insulin-like growth factor 1a receptor Kit ELISA, AARS Kit ELISA, IGF1R Kit ELISA, Igf1r Kit ELISA, igf1r Kit ELISA, Igf1 Kit ELISA, LOC780503 Kit ELISA, LOC100136427 Kit ELISA, igf1r.S Kit ELISA, igf1rb Kit ELISA, igf1ra Kit ELISA

ID gène: 16001

UniProt: Q60751

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy