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BIRC3 Kit ELISA

BIRC3 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885857
  • Antigène Voir toutes BIRC3 Kits ELISA
    BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))
    Reactivité
    • 3
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat cIAP-2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Rat cIAP-2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes BIRC3 Kits ELISA
    BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))
    Autre désignation
    cIAP-2 (BIRC3 Produits)
    Synonymes
    AIP1 Kit ELISA, API2 Kit ELISA, CIAP2 Kit ELISA, HAIP1 Kit ELISA, HIAP1 Kit ELISA, MALT2 Kit ELISA, MIHC Kit ELISA, RNF49 Kit ELISA, c-IAP2 Kit ELISA, Api1 Kit ELISA, Api2 Kit ELISA, IAP1 Kit ELISA, IAP2 Kit ELISA, MIHB Kit ELISA, Birc2 Kit ELISA, HIAP2 Kit ELISA, MIAP1 Kit ELISA, MIAP2 Kit ELISA, cIAP1 Kit ELISA, cIAP2 Kit ELISA, cIAP-1 Kit ELISA, cIAP-2 Kit ELISA, AW107670 Kit ELISA, BIRC2 Kit ELISA, baculoviral IAP repeat containing 3 Kit ELISA, baculoviral IAP repeat-containing 3 Kit ELISA, baculoviral IAP repeat-containing protein 3 Kit ELISA, inhibitor of apoptosis protein-2 Kit ELISA, BIRC3 Kit ELISA, Birc3 Kit ELISA, LOC100399118 Kit ELISA, OpmnVgp074 Kit ELISA
    UniProt
    F7FLN8
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades
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