CXCL3 Kit ELISA

N° du produit ABIN4885860

Kit ELISA CXCL3 Humain et Rat, Colorimetric test pour la quantification de Humain et Rat CXCL3.

Aperçu rapide pour CXCL3 Kit ELISA (ABIN4885860)

Antigène: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Reactivité: Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 17 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit CXCL3 Kit ELISA

Seuil minimal de détection: 17 pg/mL

Fonction: Rat CINC-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat beta-NGF, CD86 (B7-2), CINC-1 (CXCL1), CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, ICAM-1 (CD54), IFNgamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-10, IL-13, IL-2, IL-4, IL-6, LIX, L-Selectin (CD62L), MCP-1 (CCL2), PDGF-AA, Prolactin R, RAGE, TCK-1 (CXCL7), TIMP-1, TNF alpha, VEGF-A.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Autre désignation: CINC-2

Sujet:

Gene Symbols: CXCL3, CINC2

Protein Name (Synonyms): C-X-C motif chemokine 3 (Cytokine-induced neutrophil chemoattractant 2) (CINC-2) (Macrophage inflammatory protein 2-alpha/beta) (MIP2-alpha/beta)

ID gène: 171551

UniProt: Q10746

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy