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IGFBP6 Kit ELISA
IGFBP6
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885876
Détail du produit IGFBP6 Kit ELISA
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Antigène
Voir toutes IGFBP6 Kits ELISA
IGFBP6
(Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
Reactivité
Toutes les réactivités sur IGFBP6 Kits ELISA
Rat
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Rat IGFBP-6 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Rat IGFBP-6.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IGFBP6 Kits ELISA
IGFBP6
(Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
Autre désignation
IGFBP-6 (IGFBP6 Produits )
Synonymes
IBP6 Kit ELISA, IGFBP-6 Kit ELISA, IGF-BP6 Kit ELISA, RBP6A Kit ELISA, IGFBP6 Kit ELISA, igfbp6 Kit ELISA, insulin like growth factor binding protein 6 Kit ELISA, insulin-like growth factor binding protein 6 Kit ELISA, IGFBP6 Kit ELISA, Igfbp6 Kit ELISA, IGFBP-6 Kit ELISA, igfbp6 Kit ELISA
ID gène
25641
UniProt
P35572
Pathways
Signalisation WNT , Myometrial Relaxation and Contraction
Vus récemment
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