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EPH Receptor A8 Kit ELISA

EPHA8 Reactivité: Humain pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886071
  • Antigène Voir toutes EPH Receptor A8 (EPHA8) Kits ELISA
    EPH Receptor A8 (EPHA8)
    Épitope
    pTyr
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Phosphotyrosine EphA8 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA8 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA8.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes EPH Receptor A8 (EPHA8) Kits ELISA
    EPH Receptor A8 (EPHA8)
    Autre désignation
    EphA8 (EPHA8 Produits)
    Synonymes
    TK Kit ELISA, tke17-a Kit ELISA, si:ch211-122l14.1 Kit ELISA, EPHA8 Kit ELISA, EEK Kit ELISA, EK3 Kit ELISA, HEK3 Kit ELISA, AW047546 Kit ELISA, Eek Kit ELISA, Hek3 Kit ELISA, mKIAA1459 Kit ELISA, EPH receptor A8 L homeolog Kit ELISA, EPH receptor A8 Kit ELISA, eph receptor A8 Kit ELISA, Eph receptor A8 Kit ELISA, epha8.L Kit ELISA, EPHA8 Kit ELISA, epha8 Kit ELISA, Epha8 Kit ELISA
    ID gène
    2046
    UniProt
    P29322
    Pathways
    Signalisation RTK
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