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HDAC4 Kit ELISA

HDAC4 Reactivité: Humain, Souris, Rat pSer632 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886123
  • Antigène Voir toutes HDAC4 Kits ELISA
    HDAC4 (Histone Deacetylase 4 (HDAC4))
    Épitope
    pSer632
    Reactivité
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Phospho-HDAC4 (S632) Kit. This assay semi-quantitatively measures phophorylated HDAC4 (Ser632) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human/mouse/rat HDAC4 phosphorylated at site Serine-632.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product HDAC4 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes HDAC4 Kits ELISA
    HDAC4 (Histone Deacetylase 4 (HDAC4))
    Autre désignation
    HDAC4 (HDAC4 Produits)
    Synonymes
    CG1770 Kit ELISA, DHDAC4 Kit ELISA, Dmel\\CG1770 Kit ELISA, GC1770 Kit ELISA, HDAC Kit ELISA, HDAC4a Kit ELISA, dHDAC4 Kit ELISA, dmHDA405 Kit ELISA, hdac4 Kit ELISA, dana_GLEANR_19966 Kit ELISA, DanaGF18710 Kit ELISA, GF18710 Kit ELISA, HDAC4 Kit ELISA, AHO3 Kit ELISA, BDMR Kit ELISA, HA6116 Kit ELISA, HD4 Kit ELISA, HDAC-4 Kit ELISA, HDAC-A Kit ELISA, HDACA Kit ELISA, 4932408F19Rik Kit ELISA, AI047285 Kit ELISA, wu:fa96d08 Kit ELISA, wu:fc56f08 Kit ELISA, zgc:152701 Kit ELISA, histone deacetylase 4 Kit ELISA, hypothetical protein Kit ELISA, Histone deacetylase 4 Kit ELISA, GF18710 gene product from transcript GF18710-RC Kit ELISA, PTRG_03699 Kit ELISA, PGTG_15512 Kit ELISA, HDAC4 Kit ELISA, Dana\GF18710 Kit ELISA, Hdac4 Kit ELISA, hdac4 Kit ELISA
    ID gène
    25698
    UniProt
    P56524
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
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