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NLRP2 Kit ELISA

NLRP2 Reactivité: Humain pSer343 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886222
  • Antigène Voir toutes NLRP2 Kits ELISA
    NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))
    Épitope
    pSer343, total
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-NBS1 (S343) and Total NBS1 ELISA Kit. This assay semi-quantitatively measures phophorylated NBS1 (Ser343) and Total NBS1 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human NBS1 phosphorylated at site Serine-343 and total NBS1.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product NLRP2 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes NLRP2 Kits ELISA
    NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))
    Autre désignation
    NBS1 (NLRP2 Produits)
    Synonymes
    CLR19.9 Kit ELISA, NALP2 Kit ELISA, NBS1 Kit ELISA, PAN1 Kit ELISA, PYPAF2 Kit ELISA, NLRP2 Kit ELISA, E330007A02Rik Kit ELISA, Nalp2 Kit ELISA, NLR family pyrin domain containing 2 Kit ELISA, NACHT, LRR and PYD domains-containing protein 2 Kit ELISA, NLR family pyrin domain containing 7 Kit ELISA, NLR family, pyrin domain containing 2 Kit ELISA, NLRP2 Kit ELISA, LOC100301026 Kit ELISA, LOC100387349 Kit ELISA, NLRP7 Kit ELISA, Nlrp2 Kit ELISA
    ID gène
    55655
    UniProt
    Q9NX02
    Pathways
    Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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