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TYK2 Kit ELISA

TYK2 Reactivité: Humain, Souris pTyr1054 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN4886339
  • Antigène Voir toutes TYK2 Kits ELISA
    TYK2 (Tyrosine Kinase 2 (TYK2))
    Épitope
    pTyr1054, total
    Reactivité
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human and Mouse Phospho-TYK2 (Tyr1054) and Total TYK2 ELISA Kit. This assay semi-quantitatively measures phophorylated TYK2 (Tyr1054) and Total TYK2 in lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human and Mouse TYK2 phosphorylated at site Tyrosine-1054 as well as total TYK2.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product TYK2 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes TYK2 Kits ELISA
    TYK2 (Tyrosine Kinase 2 (TYK2))
    Autre désignation
    TYK2 (TYK2 Produits)
    Synonymes
    JTK1 Kit ELISA, tyrosine kinase 2 Kit ELISA, TYK2 Kit ELISA, Tyk2 Kit ELISA
    ID gène
    7297
    UniProt
    P29597, Q9R117
    Pathways
    Signalistation JAK/STAT, Hepatitis C
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