Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Caspase 7 Kit ELISA

CASP7 Reactivité: Humain, Souris Cleaved-Asp198 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4889767
  • Antigène Voir toutes Caspase 7 (CASP7) Kits ELISA
    Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
    Épitope
    Cleaved-Asp198, Uncleaved
    Reactivité
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse CASP-7 (D198) ELISA Kit. This ELISA is for measuring cleaved CASP-7 (Asp-198) as well as CASP-7 in human and mouse cell lysates.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-7 cleaved at site Aspartic Acid-198 as well as caspase-7.
    Attributs du produit
    • Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-cleaved Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling CASP7 Kit ELISA
    Top Product
    Discover our top product CASP7 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes Caspase 7 (CASP7) Kits ELISA
    Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
    Autre désignation
    Caspase-7 (CASP7 Produits)
    Synonymes
    xCaspase-7 Kit ELISA, caspase-7 Kit ELISA, caspase7 Kit ELISA, cmh-1 Kit ELISA, ice-lap3 Kit ELISA, mch3 Kit ELISA, CASP7 Kit ELISA, AI314680 Kit ELISA, CMH-1 Kit ELISA, ICE-IAP3 Kit ELISA, Mch3 Kit ELISA, mCASP-7 Kit ELISA, CASP-7 Kit ELISA, ICE-LAP3 Kit ELISA, LICE2 Kit ELISA, MCH3 Kit ELISA, Caspase-7 Kit ELISA, MCH3/SCA-2 Kit ELISA, SCA-2 Kit ELISA, caspase 7 Kit ELISA, caspase-7 Kit ELISA, caspase 7 S homeolog Kit ELISA, CASP7 Kit ELISA, xcaspase-7 Kit ELISA, casp7 Kit ELISA, casp7.S Kit ELISA, Casp7 Kit ELISA
    UniProt
    P55210
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Vous êtes ici:
Support technique