GDF15 Kit ELISA

N° du produit ABIN4986879

Ce kit ELISA Humain GDF15 est un kit ELISA Colorimetric conçu pour quantifier Humain GDF15.

Aperçu rapide pour GDF15 Kit ELISA (ABIN4986879)

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625-1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Détail du produit GDF15 Kit ELISA

Seuil minimal de détection: 15.625 pg/mL

Analytical Method: Quantitative

Specificité: Natural and recombinant Human GDF-15 Ligand

Sensibilité: 7 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Autre désignation: GDF-15

Sujet: Growth and differentiation factor-15 (GDF-15), also known as macrophage inhibitory cytokine-1(MIC-1), placental transforming growth factor-β, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-β) superfamily (1 - 3). GDF-15 is initially synthesized as a 40 kDa inactive precursor protein. It is then proteolytically cleaved to release the active C-terminal fragment, which is assembled into a disulfide-linked homodimer of 28 kDa to become biologically active GDF-15 (1, 4). In humans, GDF-15 is predominantly expressed in the placenta, with low levels in the kidney, pancreas, and prostate. However, its expression can be rapidly induced by cytokines such as interleukin-1 and TGF-β (1 - 3).GDF-15 has diverse biological functions. Early studies have shown that low serum GDF-15 levels correlate with miscarriages, indicating that it might be able to suppress inflammation in early pregnancy (5, 6). GDF-15 also plays an important role in tumorigenesis and metastasis. It has been observed that in many types of cancers, such as colorectal, breast, and prostate, the expression of GDF-15 is dramatically increased (7 - 9). Additionally, in cancer patients, serum levels of GDF-15 are elevated, which are of value in disease diagnosis and stratification (10 -12). GDF-15 is strongly induced by the tumor suppressor gene p53 and other anti-tumorigenic agents, such as the non-steroidal anti-inflammatory drugs and peroxisome proliferators-activated receptor γ. These findings suggest that GDF-15 may be a downstream target of those signaling pathways that regulate cell cycle arrest and apoptosis (13 - 15). Through the modulation of neuronal pathways important in the regulation of appetite and energy homeostasis, GDF-15 mediates cancer-induced anorexia and weight loss (16).

Pathways: SARS-CoV-2 Protein Interactome