IL23 Kit ELISA

N° du produit ABIN4986948

Détail du produit IL23 Kit ELISA

Antigène: IL23 (Interleukin 23 (IL23))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant Human IL-23 Ligand

Sensibilité: 4 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL23 (Interleukin 23 (IL23))

Autre désignation: IL-23 (IL23 Produits)

Synonymes: IL-23 Kit ELISA, p19 Kit ELISA, SGRF Kit ELISA, il23p19 Kit ELISA, interleukin 23, alpha subunit p19 Kit ELISA, interleukin 23 subunit alpha Kit ELISA, Il23a Kit ELISA, IL23A Kit ELISA

Sujet: Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12 (1-3). It is composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (3-7). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The human p19 subunit cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 170 aa mature protein. Human and mouse p19 subunits share 70 % aa sequence identity. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor β1 subunit (IL-12 Rβ1) and the IL-23-specific receptor subunit (IL-23 R) (7). IL-23 is produced by dendritic cells and macrophages in response to pathogens including certain bacteria and viruses and/or their components (3). IL-23 and IL-12 have overlapping but distinct biological activities. The IL-23 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12 (4). IL-12 drives the development of Th1 cells and induces production of IFN-γ by NK cells (3). In contrast, IL-23 has a role in the development/maintenance of a T cell subset characterized by the production of IL-17A, IL-17F, IL-6, and TNF-α (3, 4, 8). The induction of IL-17-producing T cells may involve the actions of TGF-β while their survival and expansion may be IL-23-dependent (9-11). The IL-23/IL-17 axis is an important mediator of inflammation. In mouse models, transgenic over-expression of IL-23 leads to a systemic inflammatory response (12). IL-23 effects on IL-17-producing T cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes (5, 8, 13-17). IL-23 may also play a role in increased tumor growth associated with chronic inflammation (18). In humans, IL-23 has been found upregulated in several pathologies with dysregulated immune function including psoriasis, Crohn