IL23 Kit ELISA

N° du produit ABIN6719809

N° du produit (Fournisseur): koa0524

Kit ELISA IL23 Souris, Colorimetric test pour la quantification de Souris IL23.

Aperçu rapide pour IL23 Kit ELISA (ABIN6719809)

Antigène: IL23 (Interleukin 23 (IL23))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Détail du produit IL23 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0524

Fournisseur: Rockland

Fonction: Mouse IL-23 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: (M23-S335) + (L20-A196)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.4%, 5%, 4.4%
Inter-Assay Precision (CV%): 6.2%, 6.9%, 5.1%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL23 (Interleukin 23 (IL23))

Autre désignation: IL-23

Sujet:

Background: Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).

Gene Full Name: interleukin 23, alpha subunit p19

ID gène: 83430

NCBI Accession: NP_112542

UniProt: Q9EQ14