IL-5 Kit ELISA

N° du produit ABIN4986960

Ce kit ELISA Souris IL-5 est un kit ELISA Colorimetric conçu pour quantifier Souris IL-5.

Aperçu rapide pour IL-5 Kit ELISA (ABIN4986960)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.02-1.0 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Détail du produit IL-5 Kit ELISA

Seuil minimal de détection: 0.02 ng/mL

Analytical Method: Quantitative

Specificité: Natural and recombinant Mouse IL-5 Ligand

Sensibilité: 7 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL-5

Sujet: Mouse interleukin 5 (mIL-5), also known as T-cell replacing factor, B-cell growth factor II, eosinophil differentiation factor and eosinophil colony stimulating factor, is a pleiotropic cytokine produced primarily by T cells (1-4). It supports the proliferation and differentiation of mouse, but not human, B cells, and enhances IgM, IgG1, IgA, and IgE secretion. IL-5 chemoattracts and enhances the survival and the effector functions of mature eosinophils and synergizes with various colony stimulating factors to increase eosinophil progenitor production and eosinophil expansion. Because of its diverse effects on eosinophils, IL-5 is strongly implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions (4-6). Mouse IL-5 cDNA encodes a 133 amino acid (aa) residue precursor protein containing a hydrophobic signal peptide that is cleaved to yield a 113 aa residue mature protein. Native mouse IL-5 is a disulfide-linked homodimeric 40-45 kDa glycoprotein with N- and O-linked carbohydrate chains. Mature human IL-5 is approximately 70 % identical at the amino acid level to mouse IL-5. Whereas mouse and human IL-5 are equally active on human cell lines, human IL-5 is much less active than mouse IL-5 in mouse cell assays (1-3). IL-5 is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes.

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour