CD40 Ligand Kit ELISA

N° du produit ABIN4987074

Détail du produit CD40 Ligand Kit ELISA

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25-10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant Human sCD40L Ligand

Sensibilité: 40 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: sCD40L (CD40LG Produits)

Synonymes: CD154 Kit ELISA, cd40l Kit ELISA, CD40L Kit ELISA, HIGM1 Kit ELISA, IGM Kit ELISA, IMD3 Kit ELISA, T-BAM Kit ELISA, TNFSF5 Kit ELISA, TRAP Kit ELISA, gp39 Kit ELISA, hCD40L Kit ELISA, CD40-L Kit ELISA, Cd40l Kit ELISA, Ly-62 Kit ELISA, Ly62 Kit ELISA, Tnfsf5 Kit ELISA, CD40 ligand Kit ELISA, TNF superfamily member 5 Kit ELISA, CD40LG Kit ELISA, cd40l Kit ELISA, Cd40lg Kit ELISA

Sujet: CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints